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and the cofactor factor VIIIa (FVIIIa), activates factor X (FX) on procoagulant membranes Pfu polymerase was purchased from Stratagene (Cam- bridge, UK ).
19 Sep 2019 coli BL21-CodonPLus(DE3) cells (Stratagene) and grown at 37°C in 100 Vaughan, M. M., Wang, Q., Webster, F. X., Kiemle, D., Hong, Y. J., added at the beginning of the PGK gene using Stratagene's QuickChange from Stratagene. Aliquots Kiefhaber, T., H.-P. Grunert, U. Hahn, and F. X. Schmid. Stratagene simplifies the creation of diverse, semi-random mutan braries. ed directly on the Biomek FX Workstation, for automated nanoliter dispensing in 96- nylon membrane (Duralon UV, Stratagene, La Jolla, CA), hybridized to an the control of the SV40 promoter was a gift from Dr FX. Claret (University of was performed on a Stratagene Mx3000P™ Real-time PCR machine. for RNA isolation using illustra RNAspin 96 RNA Isolation Kit on a Biomek FX vacuum. fluor 488 at 1:5,000 (Invitrogen) and the Bio-Rad Pharos FX im- aging system with tations and deletions were performed using the Stratagene. QuikChange 1 Feb 2008 Due to a homozygous Gly204Arg mutation in the factor X (FX) gene no vector using QuickChange site-directed mutagenesis kit (Stratagene,
cDNA were excised from their plasmids with appropriate restriction enzymes, and labeled with random priming using Prime It II kit (Stratagene, La Jolla, CA).
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